Shanghai Orsin Medical Technology Co., Ltd.
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EDTA blood collection tubes (purple cap) utilize dipotassium or tripotassium ethylenediaminetetraacetate (EDTA-K₂ or EDTA-K₃) as the core anticoagulant, featuring:
Precise Anticoagulation Mechanism: Chelates calcium ions (Ca²⁺) in blood to block thrombin activation, achieving complete anticoagulation while maximally preserving blood cell morphology.
Optimized Physical Design:
High-transparency PET plastic or glass tube with mechanical strength and light resistance;
Pre-filled precise anticoagulant dosage (1.5–2.2 mg EDTA/mL blood), vacuum accuracy ≤±5%.
Safety & Quality Control:
Gamma-irradiated sterilization (8–25 kGy), controlled endotoxin levels;
ISO13485/CE compliant; standardized purple cap for rapid identification.
Primarily for hematological testing, with specific applications:
| Test Category | Suitable Applications | Restrictions |
|---|---|---|
| Basic Hematology | Complete Blood Count (CBC), Hemoglobin (Hb), Blood Typing, Reticulocyte Count | Coagulation Tests (e.g., PT/APTT), Platelet Function Testing |
| Specialized Tests | Glycated Hemoglobin (HbA1c), Hemoglobin Electrophoresis, Flow Cytometry | Electrolytes (Ca²⁺/K⁺/Na⁺)/Iron Assays, Alkaline Phosphatase, Creatine Kinase |
| Molecular Biology | DNA/RNA Extraction, Pathogen Nucleic Acid Testing (with stabilizers) | PCR (EDTA may inhibit enzyme activity) |
Note: EDTA tubes cannot be used for electrolyte, trace element, or coagulation testing due to chelation interference.
| Procedure | Requirements | Key Operational Notes |
|---|---|---|
| Pre-collection | Check expiration date/vacuum integrity | Storage: 2–25°C, dark, dry conditions |
| Collection & Mixing | Gently invert 5–8 times immediately post-draw | Excessive force → hemolysis; insufficient mixing → microclots; accelerate if >25°C |
| Testing Window | Routine tests: complete within 24h | WBC count: 48h at 4°C; Platelet count: ≤24h at 4°C |
| Centrifugation | Not for direct centrifugation | Plasma separation requires gel separator (0.8–1.2 g/tube) |
IV. Precautions
Anticoagulant Risks:
Overfill (>2.2 mg/mL): RBC shrinkage/platelet swelling → morphology distortion;
Underfill: Inadequate anticoagulation → microclots → instrument obstruction.
Sample Handling:
Do not reuse tubes or mix additives;
Blood volume ≤ labeled capacity (e.g., ≤5.5 mL for 5 mL tube).
Safety:
EDTA is corrosive; rinse skin/eyes immediately with water if contacted.
EDTA-K₂ vs. EDTA-K₃?
Solubility: K₃ dissolves easier → liquid pre-filled tubes; K₂ → spray-dried plastic tubes;
Performance: Comparable anticoagulation, but K₃ excess may cause platelet satellitism (pseudothrombocytopenia).
Causes of Pseudothrombocytopenia? Solutions?
Causes: EDTA-induced platelet aggregation (~0.1% incidence) or neutrophil adhesion (satellitism);
Resolution: Recollect using sodium citrate tube.
Can tubes with microclots be used?
→ No. Microclots indicate anticoagulation failure → recollect with thorough mixing.
Suitable for glucose testing?
→ Compatible with sodium fluoride (2–4 mg/mL blood) to inhibit glycolysis; validate system compatibility.
Clinical Note: EDTA tubes remain the gold standard for CBC. For abnormal cases (e.g., suspected platelet disorders), parallel testing with alternative anticoagulants is recommended.